Regulation of the pleiotropic drug resistance transcription factors Pdr1 and Pdr3 in yeast

Aim: To understand how transcriptional factors Pdr1 and Pdr3, belonging to the pleiotropic drug resistance system, are activated, regulated after introducing chemical tox- ins cell in model organism Saccharomyces cere-visiae. Methods: Series of molecular methods were applied using different strains ofS. cerevisiae over-expressing proteins interest as a eukaryotic model. The stress in- troduced is represented by menadione. Results obtained performing protein detection analysis. Additionally, regulation DNA binding tran- scriptional activators stimulation quantified chromatin immunoprecipitation, employing epitope-tagged real-time qPCR. Results: Our results indicated higher expression levels factor, compared its homolo- gous Pdr3 treatment with yeast-cell defence system was tested against various organic solvents exclude possibility their presence potentially af- fecting results. indicate that most abundant 30 minutes from beginning treat- ment, 240 when function transcription factor faded. It appears PDR5 SNQ2 promoters, which both activated Pdr1, peaks around same time, or more precisely 40 start treatment. Conclusion: tendency reduction activa- tion menadione detected. One recognizing xenobiotic menadione, removed degradation mechanism. Given fact directly binds molecule, destruction might help cells remove toxic possible overexpressing part recognizes me- nadione alone sufficient detoxify hence produce tolerance towards